True optical microscopy

Optical Microscopy

binocular microscopes with image-erecting prisms, and the first stereomicroscope (14). Early in the twentieth century, microscope manufacturers began parfocalizing objectives, allowing the image to remain in focus when the microscopist exchanged objectives on the rotating nosepiece. In 1824, Zeiss introduced a LeChatelier-style metallograph with infinitycorrected optics, but this method of corr...

Optical-Resolution Photoacoustic Microscopy

Pure optical photoacoustic microscopy

The concept of pure optical photoacoustic microscopy(POPAM) was proposed based on optical rastering of a focused excitation beam and optically sensing the photoacoustic signal using a microring resonator fabricated by a nanoimprinting technique. After the refinements of the microring's working wavelength and in the resonator structure and mold fabrication, an ultrahigh Q factor of 3.0×10(5) was...

Ultrasound-modulated optical microscopy.

We demonstrate that microscopic imaging is feasible in ultrasound-modulated optical tomography (UOT) of soft biological tissues, using a high-frequency focused ultrasound transducer with a 75-MHz central frequency. Our experiments in tissue mimicking phantoms show that at an imaging depth of about 2 mm, an axial resolution better than 30 microm can be achieved, whereas the lateral resolution is...

Confocal optical microscopy

Confocal optical microscopy is a technique for increasing the contrast of microscope images, particularly in thick specimens. By restricting the observed volume, the technique keeps overlying or nearby scatterers from contributing to the detected signal. The price for this is that the instrument must observe only one point at a time (in the scanning laser version) or a group of separated points...